Under glucose starvation [67].de Francisco et al. BMC Genomics (2016) 17:Page 18 of

Relative expression levels for each MT gene are shown in the X-396MedChemExpress Ensartinib different histograms, after cellular treatment with different oxidative or apoptotic stressors, during exposures of 1 h (grey bars) or 24 h (black bars). Relative expression levels for each MT gene are shown in the different histograms, after cellular treatment with different abiotic stressors (pH, temperature or starvation (stv) during exposures of 3 h (grey bars), 24 h (black bars), 48 h (green bars) or 4 days (yellow bars). Each bar of the histogram corresponds to an average value ?SD of two or three independent experiments. Asterisks indicate significant differences from control at p < 0.05. Numbers on each bar indicate the corresponding average fold induction value for each treatment. A gene expression induction is considered positive when the fold-induction value obtained is > 2 (indicated by the dashed line). See Table 1 for MT gene identificationde Francisco et al. BMC Genomics (2016) 17:Page 19 ofAt present, this multi-stress character has been reported by the LOXO-195 site majority of expression analyses of MT genes under diverse abiotic stressors [68, 69]. The transcriptional induction of several MT genes by a variety of stress conditions suggests that cellular exposure to one type of stressor might lead to the acquisition of tolerance towards another different stressor. This cross-protection reported in some organisms suggests at least the partial existence of overlapping between genome responses to different types of stress [70]. This overlapping genome expression comprises general stress-responsive genes, such as heat-shock or MT genes.Under glucose starvation [67].de Francisco et al. BMC Genomics (2016) 17:Page 18 of7Fold -inductionTborMTTTborMTT4,34 *Fold-inductionTborMTT5,27 *Fold-induction81h1612,93 *5 46 5 43,1h 24h121h24h611,41 * 1,16 * 0,1,07 0,1,1,1,54 *3,17 *4,59 *24h1,47 *2PQ CAMTreatmentMDPQCAMMDPQCAM TreatmentMDTreatmentTelliMTT3,TmalaMTT2,Fold -induction2,2,Fold -induction2,13 * 1,1h 24h2 1,1h2,5 2 1,5 1 0,1,24h0,0,39 0,0,5 0 PQ CAM Treatment MD0,0,PQ CAM Treatment MDFig. 7 qRT-PCR analysis PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/27663262 of six Tetrahymena MT genes after apoptotic or oxidative stress treatments. Relative expression levels for each MT gene are shown in the different histograms, after cellular treatment with different oxidative or apoptotic stressors, during exposures of 1 h (grey bars) or 24 h (black bars). Each bar of the histogram corresponds to an average value ?SD of two or three independent experiments. Asterisks indicate significant differences from control at p < 0.05. Numbers on each bar indicate the corresponding average fold induction value for each treatment. A gene expression induction is considered positive when the fold-induction value obtained is > 2 (indicated by the dashed line). See Table 1 for MT gene identificationTborMTTTborMTTTborMTTFold -inductionFold -inductionFold -induction15 10 5pH 5 pH 5 pH 9 pH 9 4 42 Stv Stv TreatmentpH 9 3h pH 9 24h3h pH 5 24h pH48h Stv8 63h24h48h48h StvStv 4d0 pH 5 pH 5 pH 9 pH 9 4 42 Treatment Stv Stv4d Stv4dpH 5 pH 5 pH 9 pH 9 4 42TreatmentStvStvTelliMTTTamerMTTTmalaMTT-induction FoldFold -induction Fold -induction63h pH3h PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28878015 pH 5 24h pH5 4 33h3 224h 42 48h StvStv 4d4 224h48h 4d48h Stv4d Stv1pH 5 pH 5 pH 9 pH 9 4 42 Stv Stv TreatmentpH 5 pH 5 pH 9 pH 9 4 42 TreatmentStvStvpH 5 pH 5 pH 9 pH442StvStvTreatmentFig.