S available {at the

In planta Agrobacterium-mediated transformation of adult Arabidopsis thaliana plants by vacuum infiltration. Strategies Mol Biol. 82:259?66. Beilstein MA, Brinegar AE, Shippen DE. 2012. Evolution of your Arabidopsis telomerase RNA. Front Genet. 3:188. Beilstein MA, Nagalingum NS, Clements MD, Manchester SR, Mathews S. 2010. Dated molecular phylogenies indicate a Miocene origin for Arabidopsis thaliana. Proc Natl Acad Sci U S A. 107:18724?8728. Birchler JA, Veitia RA. 2007. The gene balance hypothesis: from classical genetics to modern genomics. Plant Cell 19:395?02. Blanc G, Wolfe KH. 2004. Widespread paleopolyploidy in model plant species inferred from age distributions of duplicate genes. Plant Cell 16:1667?678. Bunch JT, Bae NS, Leonardi J, Baumann P. 2005. Distinct requirements for Pot1 in limiting telomere length and keeping chromosome stability. Mol Cell Biol. 25:5567?578. Casteel DE, Zhuang S, Zeng Y, Perrino FW, Boss GR, Goulian M, Pilz RB. 2009. A DNA polymerase-alpha primase cofactor with homology to replication protein A-32 regulates DNA replication in mammalian cells. J Biol Chem. 284:5807?818. Chen LY, Majerska J, Lingner J. 2013. Molecular basis of telomere syndrome caused by CTC1 mutations. Genes Dev. 27:2099?108. Chen LY, Redon S, Lingner J. 2012. The human CST complicated is often a terminator of telomerase activity. Nature 488:540?44. Cifuentes-Rojas C, Kannan K, Tseng L, Shippen DE. 2011. Two RNA subunits and POT1a are components of Arabidopsis telomerase. Proc Natl Acad Sci U S A. 108:73?8. Cifuentes-Rojas C, Nelson AD, Boltz KA, Kannan K, She X, Shippen DE. 2012. An option telomerase RNA in Arabidopsis modulates enzyme activity in response to DNA damage. Genes Dev. 26: 2512?523. Cocciolone SM, Cone KC. 1993. Pl-Bh, an anthocyanin regulatory gene of maize that results in (-)-Blebbistatin Formula variegated pigmentation. Genetics 135: 575?88. Croy JE, Podell ER, Wuttke DS. 2006. A new model for Schizosaccharomyces pombe telomere recognition: the telomeric single-stranded DNA-binding activity of Pot11-389. J Mol Biol. 361: 80?three. Croy JE, Wuttke DS. 2006. Themes in ssDNA recognition by telomere-end protection proteins. Trends Biochem Sci. 31: 516?25. Cui L, Wall PK, Leebens-Mack JH, Lindsay BG, Soltis DE, Doyle JJ, Soltis PS, Carlson JE, Arumuganathan K, Barakat A, et al.S available at the corresponding genome portals (http://www.phytozome.net
S accessible in the corresponding genome portals (http://www.phytozome.net/; last accessed October 15, 2014) with a. thaliana POT1 proteins as a query. BLAST searches with human or S. pombe POT1 proteins had been also attempted, but did not improve the outcome. For species in Brassicaceae lacking whole-genome sequence, we developed degenerate primers to amplify either the POT1a or POT1b paralog using hiTAIL-PCR (Liu and Chen PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/26262685 2007). Sequences retrieved PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/23721119 by BLAST searches were annotated as POT1 homologs, whereas POT1 sequences resulting from amplification were deposited in GenBank. Accession numbers are supplied in supplementary table S1, Supplementary Material online.Telomere Length Evaluation and QuantificationDNA from person whole plants was extracted as described (Cocciolone and Cone 1993). TRF analysis was performed with DNA digested with Tru1I (Fermentas, Hanover, MD) restriction enzyme.

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